Description
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WNT5A in tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 98% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to WNT5A. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WNT5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT5A in the samples is then determined by comparing the O.D. of the samples to the standard curve